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KMID : 1161520070110010033
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Animal Cells and Systems
2007 Volume.11 No. 1 p.33 ~ p.38
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Effect of oral immunization with liposome-entrapped bacterial antigen on protection against experimental aeromonas hydrophila
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Choi Sang-Hoon
Oh Chan-Ho
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Abstract
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Liposome?entrapped atypical Aeromonas hydrophila antigen was prepared to investigate the potential protective efficacy for A. hydrophila infection. Carp (Cyprinus carpio) were immunized orally with liposome?entrapped A. hydrophila antigen. After immunization, significantly more antigen?specific antibodies were detected in serum, intestinal mucus and bile than non?immunized control group. The immunized carp were then challenged by immersion with 1 × 106 cfu/ml of A. hyrdophila for 60 min. Of the eight non?immunized carp, three carp died (62.5% survival), whereas five out of six (83.5%) of the immunized survived. Furthermore, development of skin ulcers was significantly inhibited in carp immunized with liposomes containing A. hydrophila antigen. These results suggest that liposomes containing A. hydrophila antigen have a potential for induction of protective immune responses against atypical A. hydrophila infection and also suggest the possibility of developing a vaccine that may ultimately be used for prevention offish diseases.
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KEYWORD
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Aeromonas hydrorphila, liposome, carp, ELISA
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